Romano N, La Licata R, Scarlata G
Infect Immun. 1977 Jun;16(3):734-7. doi: 10.1128/iai.16.3.734-737.1977.
The cell membranes of a T-strain of mycoplasma, obtained by ultrasonic disruption, were as effective as whole organisms in eliciting metabolism-inhibiting and complement-fixing antibodies. The soluble fraction separated from cell membranes by centrifugation at 35,000 X g showed a minor ability to elicit an antibody response as measured by metabolism inhibition and complement fixation tests. After a further centrifugation at 100,000 X g, the immunogenic activity of the soluble fraction was completely lost. Immunogenic determinants in mycoplasma membranes could also be demonstrated by adsorption tests; cell membranes were more effective than soluble fractions in adsorbing antibody capacity from the immune sera against whole cells. It has been shown by further experiments that cell membranes have at least two major antigenic determinants, which differ either in chemical nature or in capacity to adsorb and evoke antibodies, characterized by different serological behaviors.
通过超声破碎获得的一株支原体T株的细胞膜，在引发代谢抑制抗体和补体结合抗体方面与完整生物体一样有效。通过在35,000×g下离心从细胞膜分离出的可溶性部分，通过代谢抑制和补体结合试验测定，显示出较弱的引发抗体反应的能力。在100,000×g下进一步离心后，可溶性部分的免疫原性活性完全丧失。支原体膜中的免疫原性决定簇也可以通过吸附试验证明；在从抗全细胞免疫血清中吸附抗体能力方面，细胞膜比可溶性部分更有效。进一步的实验表明，细胞膜至少有两个主要的抗原决定簇，它们在化学性质上或吸附和诱发抗体的能力上有所不同，具有不同的血清学行为特征。
