Tomori O
Arch Virol. 1977;55(3):181-6. doi: 10.1007/BF01319904.
Monolayer cultures of cell strains derived from Cercopithicus kidney (Vero), baby hamster kidney (BHK-21), duck embryo (DE), new-born rabbit kidney (MA-111), and Aedes albopictus were tested for their ability to support the growth of Orungo virus. The virus multiplied to high titres with accompanying cytopathic effect (CPE) in Vero and BHK-21 cultures, but not in the other three culture systems. The yield of virus from infected Vero cell cultures incubated at 37 degrees C was 30 to 100 times greater than in infected cultures incubated at 23 degrees C. In addition, a CPE was observed earlier and reached completion at the higher incubating temperature. Tiny plaques 1 to 2 mm in diameter were produced only in Vero but not in BHK-21, MA-111, DE or Aedes albopictus systems.
对源自猕猴肾脏（Vero）、幼仓鼠肾脏（BHK - 21）、鸭胚（DE）、新生兔肾脏（MA - 111）以及白纹伊蚊的细胞系单层培养物进行了测试，以评估它们支持奥伦戈病毒生长的能力。该病毒在Vero和BHK - 21培养物中大量增殖并伴有细胞病变效应（CPE），但在其他三种培养系统中则不然。在37摄氏度下培养的受感染Vero细胞培养物中的病毒产量比在23摄氏度下培养的受感染培养物中的产量高30至100倍。此外，在较高培养温度下更早观察到CPE并完成。仅在Vero系统中产生了直径为1至2毫米的微小蚀斑，而在BHK - 21、MA - 111、DE或白纹伊蚊系统中未产生。
