Otoguro K, Awaya J, Tanaka H, Omura S
J Biochem. 1981 Feb;89(2):523-9. doi: 10.1093/oxfordjournals.jbchem.a133228.
Cell growth of Saccharomyces cerevisiae ATCC 12341 inhibited by the antibiotic cerulenin, a specific inhibitor of fatty acid synthesis, was restored by oleic acid (18 : 1) to give saturated fatty acid-starved cells, which could not grow when again transferred into a fresh synthetic medium containing the antibiotic and oleic acid. The growth of the saturated fatty acid-starved cells was restored when they were transferred into a medium supplemented with myristic acid (14 : 0), pentadecanoic acid (15 : 0), and palmitic acid (16 : 0) in the presence of cerulenin and oleic acid. Cellular saturated fatty acid content in the growth-restored cells was also restored to about two-thirds of that of the normal yeast cells. The DNA, RNA, and cell wall synthetic capabilities of the saturated fatty acid-starved cells were almost normal, but the L-leucine uptake and cytochrome pattern were severely impaired. These impairments were reversed on supplying palmitic acid. The decrease of L-leucine uptake of the yeasts was also caused by the addition of cerulenin alone. However, since the decrease occurred later than the inhibition of fatty acid synthesis, it was considered to be a secondary effect. These results, obtained by using the saturated fatty acid-starved cells, indicate that the membranes of S. cerevisiae require certain amounts of saturated fatty acid and that the membrane functions (energy metabolism, transport, and so on) are impaired by starvation of saturated fatty acids.
脂肪酸合成的特异性抑制剂抗生素浅蓝菌素可抑制酿酒酵母ATCC 12341的细胞生长，而油酸（18 : 1）可恢复其生长，从而产生饱和脂肪酸饥饿细胞。当再次将这些细胞转移到含有抗生素和油酸的新鲜合成培养基中时，它们无法生长。当将饱和脂肪酸饥饿细胞转移到在浅蓝菌素和油酸存在下补充了肉豆蔻酸（14 : 0）、十五烷酸（15 : 0）和棕榈酸（16 : 0）的培养基中时，其生长得以恢复。生长恢复的细胞中的细胞饱和脂肪酸含量也恢复到正常酵母细胞的约三分之二。饱和脂肪酸饥饿细胞的DNA、RNA和细胞壁合成能力几乎正常，但L-亮氨酸摄取和细胞色素模式严重受损。在供应棕榈酸后，这些损伤得到逆转。单独添加浅蓝菌素也会导致酵母L-亮氨酸摄取减少。然而，由于这种减少发生在脂肪酸合成受到抑制之后，因此被认为是一种继发效应。通过使用饱和脂肪酸饥饿细胞获得的这些结果表明，酿酒酵母的膜需要一定量的饱和脂肪酸，并且饱和脂肪酸饥饿会损害膜功能（能量代谢、转运等）。
