Rottem S, Barile M F
Antimicrob Agents Chemother. 1976 Feb;9(2):301-7. doi: 10.1128/AAC.9.2.301.
Cerulenin markedly inhibited the growth of Acholeplasma laidlawii. A. axanthum and A. granularum were less susceptible, whereas the sterol-requiring Mycoplasma species examined showed very little susceptibility. The inhibition was not reversed by the addition of long-chain fatty acids to the medium. At a concentration of 20 mug/ml, cerulenin inhibited the incorporation of [(14)C]acetate into A. laidlawii membrane lipids, but it had no effect on either protein or nucleic acid biosynthesis. Cerulenin inhibited both the de novo synthesis of long-chain fatty acids and the elongation of medium-chain fatty acids. As a result, carotenoid biosynthesis was stimulated, and increased amounts of oleic and elaidic acids were incorporated into membrane polar lipids. Our studies support the concept that cerulenin can serve as a useful tool for obtaining better control of fatty acid composition of A. laidlawii membranes.
浅蓝菌素显著抑制莱氏无胆甾原体的生长。黄无胆甾原体和颗粒无胆甾原体对其敏感性较低，而所检测的需要甾醇的支原体物种对其敏感性极低。向培养基中添加长链脂肪酸并不能逆转这种抑制作用。在浓度为20微克/毫升时，浅蓝菌素抑制了[(14)C]乙酸盐掺入莱氏无胆甾原体膜脂中，但对蛋白质或核酸生物合成没有影响。浅蓝菌素既抑制长链脂肪酸的从头合成，也抑制中链脂肪酸的延长。结果，类胡萝卜素生物合成受到刺激，并且有更多的油酸和反油酸掺入膜极性脂中。我们的研究支持这样一种观点，即浅蓝菌素可作为一种有用的工具，用于更好地控制莱氏无胆甾原体膜的脂肪酸组成。
