Perlow M, Ebert M H, Gordon E K, Ziegler M G, Lake C R, Chase T N
Brain Res. 1978 Jan 6;139(1):101-13. doi: 10.1016/0006-8993(78)90063-x.
Cerebrospinal fluid (CSF) was removed continuously in 2- or 3-h aliquots from the lateral and fourth cerebral ventricles of chronic chair restrained rhesus monkeys. Under conditions of 12 h light (06.00-18.00 h) and 12 h darkness (18.00-06.00 h) the concentrations of norepinephrine (NE) were found to describe a circadian pattern, with maximal concentrations occurring during the light hours and minimal concentrations occurring during the dark hours. The patterns were generally coincident with the circadian patterns of brain temperature and body activity. When assayed for 3-methoxy-4-hydroxyphenylethylene glycol (MHPG) and 3-methoxy-4-hydroxymandelic acid (VMA), samples of CSF collected over 3-4 days demonstrated no reproducible pattern of change. Fluctuation in the concentration of MHPG did not correspond in direction or magnitude to changes in the concentration of VMA. These random fluctuations may in part be accounted for by the influx of the metabolites from peripheral sources to the brain and CSF, and by the relatively slow movement of these metabolites as they diffuse from brain parenchyma to the CSF.
从长期处于约束椅状态的恒河猴的侧脑室和第四脑室中，每隔2至3小时连续采集脑脊液样本。在12小时光照（06:00 - 18:00时）和12小时黑暗（18:00 - 06:00时）的条件下，发现去甲肾上腺素（NE）的浓度呈现昼夜节律模式，最高浓度出现在光照时段，最低浓度出现在黑暗时段。这些模式通常与脑温和身体活动的昼夜节律模式一致。对连续3 - 4天采集的脑脊液样本进行3 - 甲氧基 - 4 - 羟基苯乙二醇（MHPG）和3 - 甲氧基 - 4 - 羟基扁桃酸（VMA）检测时，未发现可重复的变化模式。MHPG浓度的波动在方向或幅度上与VMA浓度的变化均不对应。这些随机波动部分可能是由于代谢产物从外周来源进入脑和脑脊液，以及这些代谢产物从脑实质扩散到脑脊液时相对缓慢的移动所致。
