Prell G D, Khandelwal J K, Burns R S, Green J P
Department of Pharmacology, Mount Sinai School of Medicine, University of New York, New York 10029.
Agents Actions. 1989 Mar;26(3-4):279-86. doi: 10.1007/BF01967291.
In samples of ventricular cerebrospinal fluid (CSF) that were collected from a conscious, restrained rhesus monkey at intervals of 30 90 min, levels of the histamine metabolites, tele-methylhistamine (t-MH) and tele-methylimidazoleacetic acid (t-MIAA), were determined by gas chromatography-mass spectrometry. Levels of t-MH and t-MIAA each showed time-related fluctuations. Peak and trough concentrations of t-MIAA, the product of t-MH, paralleled, but lagged about 2 h behind, the levels of t-MH. Within the first 3 h of illumination, metabolite levels increased more than 3-fold; they fell sharply within the first 3 h of darkness. Mean levels of t-MH and t-MIAA were significantly higher during periods of illumination than of darkness. Fluctuations in the levels of pros-methylimidazoleacetic acid (p-MIAA), an endogenous isomer of t-MIAA that is not a histamine metabolite, were markedly different from those of t-MH or t-MIAA; p-MIAA levels peaked only at the middle of the dark period. The time-related fluctuations in levels of t-MH and t-MIAA, but not p-MIAA, are similar to the daily rhythmic changes observed in monkey CSF for the levels of other central neurotransmitters and peptide neurohormones.
从一只清醒、受约束的恒河猴每隔30至90分钟采集的脑室脑脊液（CSF）样本中，通过气相色谱 - 质谱法测定组胺代谢物甲基组胺（t - MH）和甲基咪唑乙酸（t - MIAA）的水平。t - MH和t - MIAA的水平均呈现出与时间相关的波动。t - MIAA（t - MH的产物）的峰值和谷值浓度与t - MH的水平平行，但滞后约2小时。在光照的前3小时内，代谢物水平增加了3倍多；在黑暗的前3小时内急剧下降。t - MH和t - MIAA的平均水平在光照期间显著高于黑暗期间。甲基咪唑乙酸（p - MIAA）是t - MIAA的内源性异构体，不是组胺代谢物，其水平波动与t - MH或t - MIAA明显不同；p - MIAA水平仅在黑暗期中间达到峰值。t - MH和t - MIAA水平与时间相关的波动，而非p - MIAA的波动，类似于在恒河猴脑脊液中观察到的其他中枢神经递质和肽类神经激素水平的每日节律变化。
