正常结缔组织中非胶原网状纤维成分的抗原特性。

Antigenic properties of a non-collagenous reticulin component of normal connective tissue.

作者信息

Pras M, Johnson G D, Holborow E J, Glynn L E

出版信息

Immunology. 1974 Sep;27(3):469-78.

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1445620/

Abstract

Rabbit antisera raised against a saline-insoluble non-collagenous reticulin component (NCRC) of pig and human kidney gave immunofluorescent staining of basement membranes, stroma of liver and kidney and newly formed blood vessels in pig, rat and human tissue. The staining patterns closely resembled those reported for anti-reticulin antibodies, and both species-specific and species-shared determinants could be distinguished. Although the antisera reacted least with rat glomeruli in cryostat sections, rabbit immunoglobulin localized persistently but harmlessly in the renal glomeruli of rats given the antisera intravenously. Absorption with NCRC of sera from patients with gluten-sensitive enteropathy in most cases removed the anti-reticulin antibody characteristic of this group of diseases.

摘要

用猪和人肾脏的一种盐不溶性非胶原网状蛋白成分（NCRC）制备的兔抗血清，在猪、大鼠和人组织中，对基底膜、肝和肾的基质以及新形成的血管进行了免疫荧光染色。染色模式与报道的抗网状蛋白抗体的模式非常相似，并且可以区分物种特异性和物种共享的决定簇。尽管抗血清在冰冻切片中与大鼠肾小球的反应最弱，但将抗血清静脉注射给大鼠后，兔免疫球蛋白会持续且无害地定位于大鼠的肾小球中。在大多数情况下，用NCRC吸收麸质敏感性肠病患者的血清可去除该组疾病特有的抗网状蛋白抗体。