来自稻瘟病菌的一种细胞壁蛋白-杂聚糖：结构的进一步研究

A cell wall proteo-heteroglycan from Piricularia oryzae: further studies of the structure.

作者信息

Nakajima T, Sasaki H, Sato M, Tamari K, Matsuda K

出版信息

J Biochem. 1977 Dec;82(6):1657-62. doi: 10.1093/oxfordjournals.jbchem.a131861.

DOI:10.1093/oxfordjournals.jbchem.a131861

Abstract

The carbohydrate part of a proteo-heteroglycan from Piricularia oryzae was further studied by chemical and immunological methods. Acetolysis studies of the proteo-heteroglycan and exo-alpha-D-mannanase resistant core showed a (1 leads to 6) mannan back-bone structure with side chains composed of one to four mannose units, and some of which are terminated by D-glucose or D-galactofuranose. The mode of attachment of the terminal glucose was characterized to be alphaGlc(1 leads to 6)Man by inhibition reaction with oligosaccharides. Rabbit anti-serum formed against P. oryzae cells had three specificities, the first one for alphaGlc(1 leads to 6)alphamannosyl, the second one for alphaMan(1 leads to 3)alphamannosyl, and the last one for alphaGal-f(1 leads to 2)alphamannosyl residues. The most immunodominant side chain structure of the P. oryzae heteroglycan was shown to be alphaGlc(1 leads to 6)alphaMan(1 leads to 2)alphaMan(1 leads to 2)Man.

摘要

利用化学和免疫学方法对稻瘟病菌蛋白杂聚糖的碳水化合物部分进行了进一步研究。对该蛋白杂聚糖和外切α-D-甘露聚糖酶抗性核心进行乙酰解研究，结果显示其具有(1→6)甘露聚糖主链结构，侧链由1至4个甘露糖单元组成，其中一些侧链以D-葡萄糖或D-呋喃半乳糖终止。通过与寡糖的抑制反应，确定末端葡萄糖的连接方式为αGlc(1→6)Man。针对稻瘟病菌细胞制备的兔抗血清具有三种特异性，第一种针对αGlc(1→6)α甘露糖基，第二种针对αMan(1→3)α甘露糖基，最后一种针对αGal-f(1→2)α甘露糖基残基。结果表明，稻瘟病菌杂聚糖最具免疫优势的侧链结构为αGlc(1→6)αMan(1→2)αMan(1→2)Man。