产朊假丝酵母葡甘露聚糖的免疫化学分析。

Immunochemical analysis of the glucomannan from Candida utilis.

作者信息

Ogawa K, Matsuda K, Tamari K, Kiyo-oka S

机构信息

Department of Fundamental Science, College of Science and Engineering, Iwaki Meisei University, Fukushima, Japan.

出版信息

Agric Biol Chem. 1990 Mar;54(3):593-7.

PMID:1368530

Abstract

A glucomannan isolated from a Candida utilis mutant having a new chemotype was further studied by inhibition of the homologous precipitin reaction with oligosaccharides obtained from the glucomannan by partial acid hydrolysis and controlled acetolysis. Oligosaccharides having at least two consecutive alpha-(1----2)-linked mannose residues at the non-reducing end and gluco-manno-pentasaccharide were effective inhibitors. Thus, it appears that the glucomannan had two groups of antigenic determinants, one corresponding to the side chains of two, three, and four mannose units connected by alpha-(1----2)-linkage, and the other corresponding to a side chain composed of an O-alpha-D-glucopyranosyl-(1----6)-O-alpha-D-mannopyranosyl- (1----2)-O-alpha-D-mannopyranosyl-(1----2)-D-mannose unit. These results support a probable structure of repeating units for the glucomannan presented previously. The relative susceptibility of intersaccharidic linkages to acid hydrolysis in the mannan is discussed.

摘要

从具有新化学类型的产朊假丝酵母突变体中分离出的葡甘露聚糖，通过用部分酸水解和可控乙酰解从葡甘露聚糖获得的寡糖抑制同源沉淀反应进行了进一步研究。在非还原端具有至少两个连续的α-(1→2)-连接的甘露糖残基的寡糖和葡糖甘露五糖是有效的抑制剂。因此，似乎葡甘露聚糖有两组抗原决定簇，一组对应于通过α-(1→2)-连接连接的两个、三个和四个甘露糖单元的侧链，另一组对应于由O-α-D-吡喃葡萄糖基-(1→6)-O-α-D-吡喃甘露糖基-(1→2)-O-α-D-吡喃甘露糖基-(1→2)-D-甘露糖单元组成的侧链。这些结果支持了先前提出的葡甘露聚糖重复单元的可能结构。讨论了甘露聚糖中糖间键对酸水解的相对敏感性。