Klebanoff S J
J Exp Med. 1967 Dec 1;126(6):1063-78. doi: 10.1084/jem.126.6.1063.
Myeloperoxidase, iodide, and H(2)O(2) have a bactericidal effect on Escherichia coli. Myeloperoxidase can be replaced in this system by lactoperoxidase or by a guinea pig leukocyte particulate preparation, H(2)O(2) by an H(2)O(2)-generating system such as glucose and glucose oxidase, and iodide by thyroxine or triiodothyronine. The bactericidal effect was high at pH 5.0 and fell as the pH was increased. Preincubation of myeloperoxidase, iodide, and H(2)O(2) for 30 min before the addition of the bacteria largely prevented the bactericidal effect. Thus, the organisms must be present in the reaction mixture during iodide oxidation for maximum killing, which suggests the involvement of labile intermediates of iodide oxidation rather than the more stable end products of oxidation such as iodine. Iodination of the bacteria by the myeloperoxidase-iodide-H(2)O(2) system was demonstrated chemically and radioautographically. Iodination and the bactericidal effect were similarly affected by changes in experimental conditions in all the parameters tested (effect of preincubation, pH, and inhibitors). Phagocytosis of bacteria by guinea pig leukocytes was associated with the conversion of iodide to a trichloroacetic acid-precipitable form. Iodide was localized radioautographically in the cytoplasm of human leukocytes which contained ingested bacteria. Iodide fixation was not observed in the absence of phagocytosis or in the presence of Tapazole.
髓过氧化物酶、碘化物和H₂O₂对大肠杆菌具有杀菌作用。在该系统中，髓过氧化物酶可用乳过氧化物酶或豚鼠白细胞颗粒制剂替代，H₂O₂可用葡萄糖和葡萄糖氧化酶等H₂O₂生成系统替代，碘化物可用甲状腺素或三碘甲状腺原氨酸替代。杀菌作用在pH 5.0时较高，随着pH升高而下降。在添加细菌之前，将髓过氧化物酶、碘化物和H₂O₂预孵育30分钟可很大程度上阻止杀菌作用。因此，为实现最大程度的杀灭，碘化物氧化过程中反应混合物中必须存在细菌，这表明参与杀菌的是碘化物氧化的不稳定中间体，而非氧化的更稳定终产物，如碘。通过化学方法和放射自显影法证实了髓过氧化物酶 - 碘化物 - H₂O₂系统对细菌的碘化作用。在所有测试参数（预孵育、pH和抑制剂的影响）中，碘化作用和杀菌作用受实验条件变化的影响相似。豚鼠白细胞对细菌的吞噬作用与碘化物转化为三氯乙酸可沉淀形式有关。通过放射自显影法发现碘化物定位于含有吞噬细菌的人白细胞细胞质中。在无吞噬作用或存在他巴唑的情况下未观察到碘化物固定。
