Tomoeda M, Inuzuka M, Anto S, Konishi M
J Bacteriol. 1974 Dec;120(3):1158-63. doi: 10.1128/jb.120.3.1158-1163.1974.
Growth of Proteus mirabilis harboring R100-1 (fi(+)drd str(r)cml(r)tet(r)sul(r)) factors in Penassay broth containing sodium dodecyl sulfate (SDS) leads to the loss of all or part of the genetic elements in high frequencies. In media containing SDS at concentrations as low as 0.03%, both lysis of R(+) cells and elimination of the R factors occur at high frequencies. Appearance of drug-susceptible cells in R(+) cultures occurs during the exponential phase of growth; however, the frequencies of susceptible cells increase substantially after the culture reaches the stationary phase. Reconstruction experiments, coupled with other observations, suggest that the major factor in altering the frequency of drug-susceptible variants is the greater resistance of the variants to the lytic action of SDS. This resistance correlates in most cases with the loss of the transfer functions in the resistance transfer factor.
携带R100 - 1（fi(+)drd str(r)cml(r)tet(r)sul(r)）因子的奇异变形杆菌在含有十二烷基硫酸钠（SDS）的药敏肉汤中生长会导致全部或部分遗传元件以高频率丢失。在含有低至0.03% SDS的培养基中，R(+)细胞的裂解和R因子的消除都以高频率发生。R(+)培养物中药物敏感细胞的出现发生在生长指数期；然而，培养物进入稳定期后，敏感细胞的频率会大幅增加。重建实验以及其他观察结果表明，改变药物敏感变体频率的主要因素是变体对SDS裂解作用的更强抗性。在大多数情况下，这种抗性与抗性转移因子中转移功能的丧失相关。
