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R 因子脱氧核糖核酸分子的复合环状形式

Composite circular forms of R factor deoxyribonucleic acid molecules.

作者信息

Nisioka T, Mitani M, Clowes R

出版信息

J Bacteriol. 1969 Jan;97(1):376-85. doi: 10.1128/jb.97.1.376-385.1969.

DOI:10.1128/jb.97.1.376-385.1969
PMID:5764338
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC249615/
Abstract

Two R factors, one (R15) conferring resistance to streptomycin and sulfonamide (SM(r)SU(r)) and the other (222/R3) to streptomycin, sulfonamide, and chloramphenicol (SM(r)SU(r)CM(r)), were transferred to a Proteus mirabilis strain, and deoxyribonucleic acid (DNA) extracted from these strains was subjected to density-gradient centrifugation. R15-DNA formed a single satellite band at a density of 1.709 g cm(-3). Electron microscopy of samples from this band showed circular molecules of one type, with a contour length of 18 mum (35 x 10(6) daltons). 222/R3-DNA formed a satellite band with three peaks at densities 1.708, 1.711 and 1.717 g cm(-3). Electron micrographs revealed circular structures from each band with contour lengths, respectively, of 29 (54 x 10(6) daltons), 36 (68 x 10(6) daltons), and 6 mum (12 x 10(6) daltons). "Supertwisted" forms of several molecular species were found. It is suggested that 222/R3 DNA comprises either a single 36-mum molecule or two individual molecules, 29 and 6 mum in length, and that this may reflect the evolutionary development of R factors.

摘要

两种R因子，一种（R15）赋予对链霉素和磺胺的抗性（SM(r)SU(r)），另一种（222/R3）赋予对链霉素、磺胺和氯霉素的抗性（SM(r)SU(r)CM(r)），被转移到一株奇异变形杆菌中，并且从这些菌株中提取的脱氧核糖核酸（DNA）进行了密度梯度离心。R15-DNA在密度为1.709 g cm(-3)处形成一条单一的卫星带。对该带的样品进行电子显微镜观察显示有一种类型的环状分子，其轮廓长度为18 μm（35×10(6)道尔顿）。222/R3-DNA形成一条有三个峰的卫星带，密度分别为1.708、1.711和1.717 g cm(-3)。电子显微镜照片显示来自各带的环状结构，其轮廓长度分别为29（54×10(6)道尔顿）、36（68×10(6)道尔顿）和6 μm（12×10(6)道尔顿）。发现了几种分子种类的“超螺旋”形式。有人提出222/R3 DNA由一个单一的36-μm分子或两个长度分别为29和6 μm的单个分子组成，并且这可能反映了R因子的进化发展。

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