Mickelson M N
J Bacteriol. 1974 Nov;120(2):733-40. doi: 10.1128/jb.120.2.733-740.1974.
2,4-Dinitrophenol, dicoumarol, carbonylcyanide, m-chlorophenyl-hydrazone and pentachlorophenol all depressed aerobic molar growth yields of Streptococcus agalactiae to values equal to, or less than, those supported by substrate level phosphorylation. When the only source of energy was from substrate phosphorylation (anaerobic growth conditions), there was also a severe depression of the molar growth yield by the same four uncoupling agents. These results indicate that the effect of these agents is to uncouple both substrate and oxidative phosphorylation in S. agalactiae. Amytal inhibited glucose utilization, reduced the amount of O(2) used per mole of substrate and reduced the molar cell yield to that supported by substrate phosphorylation. Atebrin inhibited the respiration rate, but final O(2) consumed per mole of substrate was unchanged, and the respiration was coupled to biosynthesis. Rotenone had no effect on respiration, substrate utilization, or on molar growth yields.
2,4-二硝基苯酚、双香豆素、羰基氰化物、间氯苯腙和五氯苯酚均能将无乳链球菌的需氧摩尔生长产量降低至等于或低于底物水平磷酸化所支持的值。当唯一的能量来源是底物磷酸化时（厌氧生长条件），相同的四种解偶联剂也会严重降低摩尔生长产量。这些结果表明，这些试剂的作用是使无乳链球菌中的底物磷酸化和氧化磷酸化均发生解偶联。阿米妥抑制葡萄糖利用，降低每摩尔底物消耗的O₂量，并将摩尔细胞产量降低至底物磷酸化所支持的水平。阿的平抑制呼吸速率，但每摩尔底物消耗的最终O₂量不变，且呼吸与生物合成相偶联。鱼藤酮对呼吸、底物利用或摩尔生长产量均无影响。
