无乳链球菌中的支链氨基酸转运
Branched-chain amino acid transport in Streptococcus agalactiae.
作者信息
Moran J W
出版信息
Appl Environ Microbiol. 1980 Jul;40(1):25-31. doi: 10.1128/aem.40.1.25-31.1980.
Abstract
The transport of the branched-chain amino acids in Streptococcus agalactiae was characterized. Glucose-grown cells were able to utilize only glucose as an energy source for transport of L-leucine, whereas lactose-grown cells could utilize both glucose and lactose. It was determined from metabolic inhibitor studies that energy from glycolysis and substrate level phosphorylation was required for active transport. Energy was found to be coupled to transport by the action of adenosine triphosphatase and the generation of a proton motive force. The branched-chain amino acids were found to share a common transport system that may consist of multiple components.
摘要
对无乳链球菌中支链氨基酸的转运进行了表征。以葡萄糖培养的细胞仅能利用葡萄糖作为L-亮氨酸转运的能量来源，而以乳糖培养的细胞则能利用葡萄糖和乳糖。通过代谢抑制剂研究确定，糖酵解和底物水平磷酸化产生的能量是主动转运所必需的。发现能量通过三磷酸腺苷酶的作用与转运相偶联，并产生质子动力。发现支链氨基酸共享一个可能由多个组分组成的共同转运系统。
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