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抗5型腺病毒六邻体多肽抗体:检测受感染KB细胞胞质中的新生多肽。

Antibody to the type 5 adenovirus hexon polypeptide: detection of nascent polypeptides in the cytoplasm of infected KB cells.

作者信息

Stinski M F, Ginsberg H S

出版信息

Intervirology. 1974;4(4):226-36. doi: 10.1159/000149967.

DOI:10.1159/000149967
PMID:4142633
Abstract

Antibody to type 5 adenovirus hexon polypeptides reacted with the isolated hexon polypeptide chanis (monomers) but not with the native multimeric hexon capsomers (trimers). Indirect immunofluorescence detected hexon polypeptides mainly in the cytoplasm of KB cells infected with types 2 and 5 adenoviruses. Cells infected with adenovirus mutants H5ts116 or H5ts115, which are defective in the production of immunologically detectable hexons, synthesized immunologically detectable hexon polypeptides at the nonpermissive temperature.

摘要

抗5型腺病毒六邻体多肽的抗体与分离出的六邻体多肽链(单体)发生反应,但不与天然多聚体六邻体壳微粒(三聚体)发生反应。间接免疫荧光法检测到六邻体多肽主要存在于被2型和5型腺病毒感染的KB细胞的细胞质中。感染了腺病毒突变体H5ts116或H5ts115(这两种突变体在产生免疫可检测的六邻体方面存在缺陷)的细胞,在非允许温度下合成了免疫可检测的六邻体多肽。

相似文献

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Antibody to the type 5 adenovirus hexon polypeptide: detection of nascent polypeptides in the cytoplasm of infected KB cells.抗5型腺病毒六邻体多肽抗体:检测受感染KB细胞胞质中的新生多肽。
Intervirology. 1974;4(4):226-36. doi: 10.1159/000149967.
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