Inderlied C B, Delwiche E A
J Bacteriol. 1973 Jun;114(3):1206-12. doi: 10.1128/jb.114.3.1206-1212.1973.
Veillonella alcalescens, a strict anaerobe, was found to possess a nitrate reductase system which has characteristics of both assimilatory and respiratory nitrate reduction. The nitrate reductase has been identified tentatively as a particulate enzyme which utilizes a variety of electron donors for the reduction of nitrate. By use of (15)N-labeled nitrate, it was shown that under appropriate conditions nitrate nitrogen is incorporated into cell material. V. alcalescens grown on pyruvate and nitrate has a greater growth rate than cells grown on pyruvate alone. Growth can occur in a medium with hydrogen and nitrate as the sole energy source. Ammonium chloride decreases the rate of nitrate reduction but does not completely inhibit reduction or incorporation. The results suggest that nitrate assimilation and respiration are not as distinct as in some other organisms.
产碱韦荣球菌是一种严格厌氧菌，已发现其拥有一种硝酸盐还原酶系统，该系统具有同化性硝酸盐还原和呼吸性硝酸盐还原的特征。硝酸盐还原酶初步鉴定为一种颗粒酶，它利用多种电子供体来还原硝酸盐。通过使用（15）N标记的硝酸盐表明，在适当条件下，硝酸盐氮会掺入细胞物质中。在丙酮酸和硝酸盐上生长的产碱韦荣球菌比仅在丙酮酸上生长的细胞具有更高的生长速率。在以氢气和硝酸盐作为唯一能源的培养基中也能生长。氯化铵会降低硝酸盐还原速率，但不会完全抑制还原或掺入。结果表明，硝酸盐同化和呼吸作用不像在其他一些生物体中那样截然不同。
