VALENTINE R C, WOLFE R S
J Bacteriol. 1963 May;85(5):1114-20. doi: 10.1128/jb.85.5.1114-1120.1963.
Valentine, R. C. (University of Illinois, Urbana) and R. S. Wolfe. Role of ferredoxin in the metabolism of molecular hydrogen. J. Bacteriol. 85:1114-1120. 1963.-The metabolism of molecular hydrogen by Clostridium pasteurianum, Micrococcus lactilyticus (Veillonella alcalescens), and several other anaerobic bacteria was studied. Oxidation of hydrogen, using several electron-accepting substrates including triphosphopyridine nucleotide, uric acid, xanthine, nitrite, and hydroxylamine, required ferredoxin in conjunction with hydrogenase. Evolution of hydrogen from pyruvate, alpha-ketoglutarate, hypoxanthine, and dithionite was mediated by ferredoxin. On the basis of these findings, a unitary hypothesis for biological hydrogen evolution is proposed in which ferredoxin plays a key role.
瓦伦丁，R.C.（伊利诺伊大学厄巴纳分校）和R.S.沃尔夫。铁氧化还原蛋白在分子氢代谢中的作用。《细菌学杂志》85:1114 - 1120。1963年。——对巴氏梭菌、乳酸微球菌（产碱韦荣球菌）及其他几种厌氧菌的分子氢代谢进行了研究。利用包括三磷酸吡啶核苷酸、尿酸、黄嘌呤、亚硝酸盐和羟胺在内的几种电子受体底物进行氢的氧化，需要铁氧化还原蛋白与氢化酶协同作用。丙酮酸、α - 酮戊二酸、次黄嘌呤和连二亚硫酸盐产生氢是由铁氧化还原蛋白介导的。基于这些发现，提出了一个关于生物放氢的统一假说，其中铁氧化还原蛋白起关键作用。
