Wisdom C, Welker N E
J Bacteriol. 1973 Jun;114(3):1336-45. doi: 10.1128/jb.114.3.1336-1345.1973.
Protoplasts of Bacillus stearothermophilus NCA 1503-4R are resistant to osmotic rupture and are not sensitive to mechanical manipulation. Protoplast stability is maintained by divalent cations. The thermostability of protoplasts is enhanced when the cells are grown at elevated temperatures. The membrane content of the cell and the protein-to-lipid ratio of the membrane increases as the growth temperature is increased. The membrane-bound nicotinamide adenine dinucleotide (reduced form) oxidase system from cultures grown at 70 C was more thermostable than the same enzyme system from cultures grown at 55 C. Alkaline phosphatase was resistant to thermal inactivation in the intact protoplast. The extent of this protection is dependent on protoplast stability.
嗜热脂肪芽孢杆菌NCA 1503 - 4R的原生质体对渗透破裂具有抗性，并且对机械操作不敏感。原生质体的稳定性由二价阳离子维持。当细胞在较高温度下生长时，原生质体的热稳定性会增强。随着生长温度的升高，细胞的膜含量以及膜的蛋白质与脂质比率会增加。在70℃培养的培养物中，膜结合的烟酰胺腺嘌呤二核苷酸（还原型）氧化酶系统比在55℃培养的培养物中的相同酶系统更耐热。碱性磷酸酶在完整的原生质体中对热失活具有抗性。这种保护的程度取决于原生质体的稳定性。
