来自嗜热水栖菌T351菌株的一种可溶性NADH脱氢酶（NADH：铁氰化物氧化还原酶）。
A soluble NADH dehydrogenase (NADH: ferricyanide oxidoreductase) from Thermus aquaticus strain T351.
作者信息
Walsh K A, Daniel R M, Morgan H W
出版信息
Biochem J. 1983 Feb 1;209(2):427-33. doi: 10.1042/bj2090427.
Abstract
A soluble NADH dehydrogenase (NADH:ferricyanide oxidoreductase) has been obtained by simple disruption of cells of Thermus aquaticus strain T351, and purified. The enzyme is of low molecular mass, 50 000 Da, and displays many of the properties of the membrane-bound enzyme, including inhibition by both NADH and ferricyanide, and the same Km for ferricyanide. The enzyme contains 0.05 mol of FMN, 0.16 mol of labile sulphur and 2.2 mol of iron per mol of protein. The enzyme is inhibited by NAD and cupferron competitively with ferricyanide, and by ATP (but not ADP) competitively with NADH. The enzyme is particularly thermostable, having a half-life at 95 degrees C of 35 min. The effect of temperature on the molar absorption coefficient and the stability of NADH was determined.
摘要
通过简单破碎嗜热水栖热菌T351菌株的细胞，获得了一种可溶性NADH脱氢酶（NADH：铁氰化物氧化还原酶）并进行了纯化。该酶分子量较低，为50000道尔顿，具有许多膜结合酶的特性，包括受NADH和铁氰化物抑制，以及对铁氰化物具有相同的米氏常数。每摩尔蛋白质中，该酶含有0.05摩尔的FMN、0.16摩尔的不稳定硫和2.2摩尔的铁。该酶被NAD和铜铁试剂与铁氰化物竞争性抑制，被ATP（而非ADP）与NADH竞争性抑制。该酶具有特别的热稳定性，在95℃下的半衰期为35分钟。测定了温度对摩尔吸收系数和NADH稳定性的影响。
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1
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2
Purification of an NADH-(dichlorophenol-indophenol) oxidoreductase from Bacillus stearothermophilus.嗜热脂肪芽孢杆菌中NADH-（二氯酚靛酚）氧化还原酶的纯化
Biochem J. 1980 Nov 1;191(2):457-65. doi: 10.1042/bj1910457.
3
Relation of Arrhenius discontinuities of NADH dehydrogenase to change in membrane lipid fluidity of Bacillus caldotenax.嗜热栖热放线菌NADH脱氢酶的阿累尼乌斯不连续性与膜脂流动性变化的关系
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