Tierno P M, Stotzky G
Ann Clin Lab Sci. 1978 Jan-Feb;8(1):42-9.
Electrophoretic and serologic characterization of extracellular proteinases from 111 isolates (80 biotype 1 and 31 biotype 4) of Staphylococcus epidermidis (coagulase negative) of human origin were conducted. Seventy-seven (60 of biotype 1, 17 of biotype 4) of the 111 proteinase-positive isolated of S. epidermidis were classified by serological differentiation of their proteolytic enzymes with known specific antisera. Thirty-two (18 of biotype 1, 14 of biotype 4 (isolates of S. epidermidis produced proteolytic enzymes that did not react with any of the known group antisera and were classified as NR (non-reacting). No classification of the enzymes of two other isolates was possible. The NR proteinase enzymes from the 32 cultures that could not be classified by serological differentiation with the known specific antisera fitted into one of three electrophoretic mobility patterns: NR1, NR2 or NR3. Seventy-eight isolates of S. epidermidis biotype 1 were placed into four proteinase sero-groups; 49 in F, 11 in B, 11 in NR2, and 7 in NR3. Thirty-one isolates of S. epidermidis biotype 4 were placed into one of two groups: 17 in C and 14 in NR. All enzymes tested migrated towards the anode at pH 8.6, and the order of electrophoretic mobility was NR3 greater than F greater than NR2 greater than NR1 greater than B greater than C.
对来源于人类的111株表皮葡萄球菌（凝固酶阴性）（80株生物型1和31株生物型4）的细胞外蛋白酶进行了电泳和血清学特性分析。在111株蛋白酶阳性的表皮葡萄球菌分离株中，77株（生物型1的60株，生物型4的17株）通过用已知特异性抗血清对其蛋白水解酶进行血清学区分来分类。32株（生物型1的18株，生物型4的14株）表皮葡萄球菌分离株产生的蛋白水解酶不与任何已知的组抗血清反应，被归类为NR（无反应）。另外两株分离株的酶无法分类。来自32种培养物的NR蛋白酶，不能通过与已知特异性抗血清的血清学区分进行分类，它们符合三种电泳迁移模式之一：NR1、NR2或NR3。78株生物型1的表皮葡萄球菌被分为四个蛋白酶血清组；49株在F组，11株在B组，11株在NR2组，7株在NR3组。31株生物型4的表皮葡萄球菌被分为两组之一：17株在C组，14株在NR组。所有测试的酶在pH 8.6时都向阳极迁移，电泳迁移顺序为NR3大于F大于NR2大于NR1大于B大于C。
