肺炎球菌摄取过程中细胞表面脱氧核糖核酸的结构
Structure of deoxyribonucleic acid on the cell surface during uptake by pneumococcus.
作者信息
Morrison D A, Guild W R
出版信息
J Bacteriol. 1973 Sep;115(3):1055-62. doi: 10.1128/jb.115.3.1055-1062.1973.
Abstract
We exposed competent cells of Diplococcus pneumoniae to high-molecular-weight donor deoxyribonucleate (DNA) and examined the state of the DNA bound to them in forms sensitive to deoxyribonuclease I. The portion elutable with 5 M guanidine hydrochloride was shown to be native, of much lower molecular weight (4 x 10(6) to 5 x 10(6)) than the donor, and as active in further transformation as sheared DNA of the same size. The portion resistant to release by guanidine hydrochloride was also shown to be native and active in transformation. These results, along with previous ones, imply that the breaks produced outside the cell are not at genetically specific sites. Furthermore, it was found that entry past the cell barrier to deoxyribonuclease could occur at 0 C by a process sensitive to ethylenediaminetetraacetate.
摘要
我们将肺炎双球菌感受态细胞暴露于高分子量的供体脱氧核糖核酸（DNA）中，并以对脱氧核糖核酸酶I敏感的形式检查与之结合的DNA的状态。用5M盐酸胍可洗脱的部分显示为天然的，分子量比供体低得多（4×10⁶至5×10⁶），并且在进一步转化中与相同大小的剪切DNA一样活跃。对盐酸胍释放有抗性的部分也显示为天然的且在转化中具有活性。这些结果与先前的结果一起表明，在细胞外产生的断裂并非发生在基因特异性位点。此外，还发现脱氧核糖核酸酶在0℃时可通过对乙二胺四乙酸敏感的过程穿过细胞屏障进入细胞。
相似文献
1
Structure of deoxyribonucleic acid on the cell surface during uptake by pneumococcus.
J Bacteriol. 1973 Sep;115(3):1055-62. doi: 10.1128/jb.115.3.1055-1062.1973.
2
Transformation and deoxyribonucleic acid size: extent of degradation on entry varies with size of donor.
J Bacteriol. 1972 Dec;112(3):1157-68. doi: 10.1128/jb.112.3.1157-1168.1972.
3
Adenosine triphosphate-dependent deoxyribonuclease from Diplococcus pneumoniae: fate of transforming deoxyribonucleic acid in a strain deficient in the enzymatic activity.
J Bacteriol. 1973 Feb;113(2):718-23. doi: 10.1128/jb.113.2.718-723.1973.
4
Breakage prior to entry of donor DNA in Pneumococcus transformation.
Biochim Biophys Acta. 1973 Apr 11;299(4):545-56. doi: 10.1016/0005-2787(73)90226-8.
5
Early stages in DNA binding and uptake during genetic transformation of pneumococci.
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1493-8. doi: 10.1073/pnas.71.4.1493.
6
Nucleolytic degradation of homologous and heterologous deoxyribonucleic acid molecules at the surface of competent pneumococci.
J Bacteriol. 1975 May;122(2):676-85. doi: 10.1128/jb.122.2.676-685.1975.
7
Kinetic analysis of the products of donor deoxyribonucleate in transformed cells of Bacillus subtilis.
J Bacteriol. 1973 Oct;116(1):154-62. doi: 10.1128/jb.116.1.154-162.1973.
8
Single-strand breakage on binding of DNA to cells in the genetic transformation of Diplococcus pneumoniae.
J Mol Biol. 1976 Feb 25;101(2):255-75. doi: 10.1016/0022-2836(76)90376-4.
9
Transformation in pneumococcus: existence and properties of a complex involving donor deoxyribonucleate single strands in eclipse.
J Bacteriol. 1977 Nov;132(2):576-83. doi: 10.1128/jb.132.2.576-583.1977.
10
Fate of transforming deoxyribonucleic acid after uptake by competent Bacillus subtilis: size and distribution of the integrated donor segments.
J Bacteriol. 1972 Aug;111(2):488-94. doi: 10.1128/jb.111.2.488-494.1972.
引用本文的文献
1
From isotopically labeled DNA to fluorescently labeled dynamic pili: building a mechanistic model of DNA transport to the cytoplasmic membrane.
Microbiol Mol Biol Rev. 2024 Mar 27;88(1):e0012523. doi: 10.1128/mmbr.00125-23. Epub 2024 Mar 11.
2
Competence in and Close Commensal Relatives: Mechanisms and Implications.
Front Cell Infect Microbiol. 2019 Apr 3;9:94. doi: 10.3389/fcimb.2019.00094. eCollection 2019.
3
Regulation of hypercompetence in Legionella pneumophila.
J Bacteriol. 2004 Jun;186(12):3814-25. doi: 10.1128/JB.186.12.3814-3825.2004.
4
Enhancement of pneumococcal transfection by protamine sulfate.
J Bacteriol. 1982 Nov;152(2):765-72. doi: 10.1128/jb.152.2.765-772.1982.
5
Monomer plasmid DNA transforms Streptococcus pneumoniae.
Mol Gen Genet. 1981;181(1):57-62. doi: 10.1007/BF00339005.
6
Isolation and characterization of three new classes of transformation-deficient mutants of Streptococcus pneumoniae that are defective in DNA transport and genetic recombination.
J Bacteriol. 1983 Oct;156(1):281-90. doi: 10.1128/jb.156.1.281-290.1983.
7
Isolation of transformation-deficient Streptococcus pneumoniae mutants defective in control of competence, using insertion-duplication mutagenesis with the erythromycin resistance determinant of pAM beta 1.
J Bacteriol. 1984 Sep;159(3):870-6. doi: 10.1128/jb.159.3.870-876.1984.
8
Acid-soluble breakdown of homologous deoxyribbonucleic acid adsorbed by Haemophilus influenzae: its biological significance.
J Bacteriol. 1974 Nov;120(2):917-22. doi: 10.1128/jb.120.2.917-922.1974.
9
Role of a deoxyribonuclease in the genetic transformation of Diplococcus pneumoniae.
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2305-9. doi: 10.1073/pnas.71.6.2305.
10
Early stages in DNA binding and uptake during genetic transformation of pneumococci.
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1493-8. doi: 10.1073/pnas.71.4.1493.
本文引用的文献
1
ENZYMIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. 18. THE REPAIR OF PARTIALLY SINGLE-STRANDED DNA TEMPLATES BY DNA POLYMERASE.
J Mol Biol. 1964 Jul;9:46-69. doi: 10.1016/s0022-2836(64)80090-5.
2
The bouyant behavior of viral and bacterial DNA in alkaline CsCl.
Proc Natl Acad Sci U S A. 1963 Jan 15;49(1):12-7. doi: 10.1073/pnas.49.1.12.
3
Ionizing radiation and ultrasonic evidence for a minimum unit of transforming principle DNA.
Biochim Biophys Acta. 1957 Nov;26(2):241-51. doi: 10.1016/0006-3002(57)90002-1.
4
A restriction enzyme from Hemophilus influenzae. II.
J Mol Biol. 1970 Jul 28;51(2):393-409. doi: 10.1016/0022-2836(70)90150-6.
5
Conversion of Bacillus subtilis DNA to phage DNA following mitomycin C induction.
J Mol Biol. 1968 Jun 28;34(3):429-37. doi: 10.1016/0022-2836(68)90170-8.
6
Bacterial inactivation of transforming deoxyribonucleate.
J Bacteriol. 1971 Sep;107(3):889-99. doi: 10.1128/jb.107.3.889-899.1971.
7
The 5'-ends of the DNA of defective bacteriophages of Bacillus subtilis.
J Mol Biol. 1970 Feb 14;47(3):591-3. doi: 10.1016/0022-2836(70)90326-8.
8
A specific complex between a restriction endonuclease and its DNA substrate.
Proc Natl Acad Sci U S A. 1970 Feb;65(2):357-62. doi: 10.1073/pnas.65.2.357.
9
Host specificity of DNA produced by Escherichia coli, X. In vitro restriction of phage fd replicative form.
Proc Natl Acad Sci U S A. 1968 Apr;59(4):1300-6. doi: 10.1073/pnas.59.4.1300.
10
Fate of transforming DNA following uptake by competent Bacillus subtilis. Formation and properties of products isolated from transformed cells which are derived entirely from donor DNA.
J Mol Biol. 1972 Feb 28;64(1):9-29. doi: 10.1016/0022-2836(72)90318-x.
Zotero 插件