Yuan R, Meselson M
Proc Natl Acad Sci U S A. 1970 Feb;65(2):357-62. doi: 10.1073/pnas.65.2.357.
In the presence of Mg(++), ATP, and S-adenosylmethionine, the DNA restriction endonuclease R.K forms a specific complex with its DNA substrate. The complex can be detected by its retention on nitrocellulose membranes.
在镁离子(Mg(++))、三磷酸腺苷(ATP)和S-腺苷甲硫氨酸存在的情况下,DNA限制性内切酶R.K与其DNA底物形成一种特异性复合物。该复合物可通过其在硝酸纤维素膜上的滞留来检测。