感受态枯草芽孢杆菌摄取转化脱氧核糖核酸后的命运：整合供体片段的大小和分布
Fate of transforming deoxyribonucleic acid after uptake by competent Bacillus subtilis: size and distribution of the integrated donor segments.
作者信息
Dubnau D, Cirigliano C
出版信息
J Bacteriol. 1972 Aug;111(2):488-94. doi: 10.1128/jb.111.2.488-494.1972.
Abstract
A series of sheared donor-recipient complex samples were prepared from a competent Bacillus subtilis culture which had been transformed by using (3)H, (2)H-labeled deoxyribonucleic acid. Measurement of the molecular weights and buoyant densities of these samples led to the following conclusions. (i) The average size of the single-stranded donor segment which is integrated is 2.8 x 10(6). (ii) These donor segments are not randomly distributed in the recipient genome, but are clustered in groups, each group containing several discrete donor segments.
摘要
从用（3）H、（2）H标记的脱氧核糖核酸转化的感受态枯草芽孢杆菌培养物中制备了一系列剪切的供体-受体复合物样品。对这些样品的分子量和浮力密度的测量得出了以下结论。（i）整合的单链供体片段的平均大小为2.8×10⁶。（ii）这些供体片段不是随机分布在受体基因组中，而是成组聚集，每组包含几个离散的供体片段。
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