两个枯草芽孢杆菌染色体基因在大肠杆菌中的功能表达。
Functional expression of two Bacillus subtilis chromosomal genes in Escherichia coli.
作者信息
Chi N Y, Ehrlich S D, Lederberg J
出版信息
J Bacteriol. 1978 Feb;133(2):816-21. doi: 10.1128/jb.133.2.816-821.1978.
Abstract
EcoRI-cleaved deoxyribonucleic acid segments carrying two genes from Bacillus subtilis, pyr and leu, have been cloned in Escherichia coli by insertion into a derivative of the E. coli bacteriophage lambda. Lysogenization of pyrimidine- and leucine-requiring auxotrophs of E. coli by the hybrid phages exhibited prototrophic phenotypes, suggesting the expression of B. subtilis genes in E. coli. Upon induction, these lysogens produced lysates capable of transducing E. coli pyr and leu auxotrophs to prototrophy with high frequency. Isolated DNAs of these bacteriophages have the ability to transform B. subtilis auxotrophs to pyr and leu independence and contain EcoRI-cleaved segments which hybridize to corresponding segments of B. subtilis.
摘要
携带枯草芽孢杆菌两个基因（pyr和leu）的经EcoRI酶切的脱氧核糖核酸片段，已通过插入大肠杆菌噬菌体λ的衍生物中，克隆到大肠杆菌中。杂种噬菌体对需要嘧啶和亮氨酸的大肠杆菌营养缺陷型进行溶源化，表现出原养型表型，这表明枯草芽孢杆菌基因在大肠杆菌中得到了表达。诱导后，这些溶源菌产生的裂解物能够高频地将大肠杆菌的pyr和leu营养缺陷型转导为原养型。这些噬菌体的分离DNA能够将枯草芽孢杆菌营养缺陷型转化为对pyr和leu不依赖型，并且含有与枯草芽孢杆菌相应片段杂交的EcoRI酶切片段。
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