枯草芽孢杆菌噬菌体Phi-3-T胸苷酸合成酶基因在大肠杆菌中的表达
Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli.
作者信息
Ehrlich S D, Bursztyn-Pettegrew H, Stroynowski I, Lederberg J
出版信息
Proc Natl Acad Sci U S A. 1976 Nov;73(11):4145-9. doi: 10.1073/pnas.73.11.4145.
Abstract
The thymidylate synthetase gene of B. subtilis bacteriophage Phi-3-T, when cloned in plasmids pSC101 or pMB9 is expressed in E. coli. The promoter of the cloned gene is likely to originate in Phi-3-T. Rearrangements of hybrid plasmid sequences during the cloning have been noted. B. subtilis strains can be transformed with hybrid DNAs. The transformants contain sequences of Phi-3-T, but not those of plasmid vectors.
摘要
枯草芽孢杆菌噬菌体Phi-3-T的胸苷酸合成酶基因，当克隆到质粒pSC101或pMB9中时，能在大肠杆菌中表达。克隆基因的启动子可能源自Phi-3-T。在克隆过程中已注意到杂交质粒序列的重排。枯草芽孢杆菌菌株可用杂交DNA进行转化。转化子含有Phi-3-T的序列，但不含有质粒载体的序列。
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