用于在航天器上检测真菌的培养基的比较。
Comparison of media for detection of fungi on spacecraft.
作者信息
Herring C M, Brandsberg J W, Oxborrow G S, Puleo J R
出版信息
Appl Microbiol. 1974 Mar;27(3):566-9. doi: 10.1128/am.27.3.566-569.1974.
Five media, including Trypticase soy agar (TSA; BBL) pour plates, spread plates of TSA, Mycophil agar with chloromycetin, Mycophil agar with chloromycetin and Actidione, and cornmeal agar with chloromycetin were quantitatively and qualitatively compared for the detection of fungi on spacecraft. Cornmeal agar with chloromycetin yielded the highest number of fungal colonies, although not always significantly higher than Mycophil agar with chloromycetin or TSA spread plates. Cornmeal agar with chloromycetin also gave the best qualitative representation of fungi on the spacecraft, recovering 68% of the genera found from all media. This medium yielded 10 times the number of fungal colonies and 3 times the number of genera found on TSA pour plates as currently used for spacecraft assay.
对包括胰蛋白胨大豆琼脂（TSA；BBL）倾注平板、TSA涂布平板、含氯霉素的麦芽汁琼脂、含氯霉素和放线菌酮的麦芽汁琼脂以及含氯霉素的玉米粉琼脂在内的五种培养基进行了定量和定性比较，以检测航天器上的真菌。含氯霉素的玉米粉琼脂产生的真菌菌落数量最多，尽管并不总是显著高于含氯霉素的麦芽汁琼脂或TSA涂布平板。含氯霉素的玉米粉琼脂在定性方面也能最好地呈现航天器上的真菌，从所有培养基中分离出的属中，它能检出68%。这种培养基产生的真菌菌落数量是目前用于航天器检测的TSA倾注平板的10倍，检出的属数量是其3倍。
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