Favero M S, Puleo J R, Marshall J H, Oxborrow G S
Appl Microbiol. 1966 Jul;14(4):539-51. doi: 10.1128/am.14.4.539-551.1966.
The primary objective of this study was to determine quantitatively and qualitatively the predominant types of microbial contamination occurring in conventional and laminar flow clean rooms. One horizontal laminar flow, three conventional industrial clean rooms, and three open factory areas were selected for microbiological tests. The results showed that as the environment and personnel of a clean room were controlled in a more positive manner with respect to the reduction of particulate contamination, the levels of airborne and surface microbial contaminants were reduced accordingly. The chief sources of microbial contamination were associated with the density and activity of clean room personnel. In addition, the majority of microorganisms isolated from the intramural air by air samplers were those indigenous to humans. Studies on the fallout and accumulation of airborne microorganisms on stainless-steel surfaces showed that, although there were no significant differences in the levels of microbial contamination among the conventional clean rooms, the type of microorganism detected on stainless-steel surfaces was consistently and significantly different. In addition, the "plateau phenomenon" occurred in all environments studied. It was concluded that the stainless-steel strip method for detecting microbial accumulation on surfaces is efficient and sensitive in ultra-clean environments and is the most reliable and practical method for monitoring microbial contamination in future class 100 clean rooms to be used for the assembly of spacecraft which will be sterilized.
本研究的主要目的是对传统洁净室和层流洁净室中存在的主要微生物污染类型进行定量和定性测定。选择了一个水平层流洁净室、三个传统工业洁净室和三个开放式工厂区域进行微生物检测。结果表明，随着洁净室的环境和人员在减少颗粒污染方面得到更积极的控制，空气传播和表面微生物污染物的水平相应降低。微生物污染的主要来源与洁净室人员的密度和活动有关。此外，通过空气采样器从室内空气中分离出的大多数微生物是人类固有的。对不锈钢表面空气传播微生物的沉降和积累的研究表明，尽管传统洁净室之间的微生物污染水平没有显著差异，但在不锈钢表面检测到的微生物类型始终存在显著差异。此外，在所研究的所有环境中都出现了“平台现象”。得出的结论是，用于检测表面微生物积累的不锈钢条法在超洁净环境中高效且灵敏，是未来用于组装将进行灭菌的航天器的100级洁净室中监测微生物污染的最可靠和实用的方法。
