将核糖中的标记掺入噬菌体SP15 DNA的5-(4',5'-二羟基戊基)尿嘧啶中。
Incorporation of label from ribose into 5-(4',5'-dihydroxypentyl) uracil of bacteriophage SP15 DNA.
作者信息
Walker M S, Mandel M
出版信息
J Virol. 1978 Feb;25(2):695-7. doi: 10.1128/JVI.25.2.695-697.1978.
Abstract
Radioactively labeled ribose was incorporated into the glucosylated deoxynucleoside monophosphate of 5-(4',5'-dihydroxypentyl)uracil of bacteriophage SP15 DNA to a greater extent than into the other pyrimidine deoxynucleoside monophosphates. Results from formic acid hydrolysis of the deoxynucleoside monophosphates to their bases suggest that label from ribose is incorporated into the dihydroxypentyl side chain of 5-(4',5'-dihydroxypentyl)uracil.
摘要
放射性标记的核糖掺入噬菌体SP15 DNA的5-(4',5'-二羟基戊基)尿嘧啶的糖基化脱氧核苷单磷酸中的程度，比掺入其他嘧啶脱氧核苷单磷酸中的程度更大。脱氧核苷单磷酸经甲酸水解为其碱基的结果表明，核糖的标记掺入了5-(4',5'-二羟基戊基)尿嘧啶的二羟基戊基侧链中。
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