噬菌体SP15感染的枯草芽孢杆菌中5-(4'5'-二羟基戊基)尿嘧啶作为核苷三磷酸的生物合成。
Biosynthesis of 5-(4'5'-dihydroxypentyl) uracil as a nucleoside triphosphate in bacteriophage SP15-infected Bacillus subtilis.
作者信息
Walker M S, Mandel M
出版信息
J Virol. 1978 Feb;25(2):500-9. doi: 10.1128/JVI.25.2.500-509.1978.
Abstract
The nucleoside triphosphate of 5-(4',5'-dihydroxypentyl)uracil (DHPU) was detected in the acid-soluble extract from bacteriophage SP15-infected Bacillus subtilis W23. No uracil was found in the DNA of either replicating or mature phage. Labeled thymidine added during phage DNA synthesis was incorporated into phage DNA. The presence of DHPU as a nucleoside triphosphate in the acid-soluble pool and the incorporation of thymidine into phage DNA suggest that both DHPU and thymine are incorporated into SP15 DNA via their nucleoside triphosphates. 5-Fluorodeoxyuridine inhibited biosynthesis of SP15 DNA, and this inhibition was reversed by thymidine, resulting in the synthesis of a DNA containing reduced amounts of fully modified DHPU. It is proposed that 5-fluorodeoxyuridine, or its metabolic product, inhibits a step in the biosynthetic pathway to the nucleoside triphosphate of DHPU.
摘要
在噬菌体SP15感染的枯草芽孢杆菌W23的酸溶性提取物中检测到了5-(4',5'-二羟基戊基)尿嘧啶（DHPU）的核苷三磷酸。在复制或成熟噬菌体的DNA中均未发现尿嘧啶。在噬菌体DNA合成过程中添加的标记胸腺嘧啶被整合到噬菌体DNA中。酸溶性池中存在作为核苷三磷酸的DHPU以及胸腺嘧啶掺入噬菌体DNA表明，DHPU和胸腺嘧啶均通过其核苷三磷酸被整合到SP15 DNA中。5-氟脱氧尿苷抑制SP15 DNA的生物合成，这种抑制作用可被胸腺嘧啶逆转，从而导致合成含有减少量的完全修饰的DHPU的DNA。有人提出，5-氟脱氧尿苷或其代谢产物会抑制DHPU核苷三磷酸生物合成途径中的一个步骤。
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