Akesson I, Haugen H, Enger E
Scand J Clin Lab Invest. 1978 Feb;38(1):93-5. doi: 10.3109/00365517809108409.
As a replacement of current time consuming techniques, a radial immunodiffusion method which is suitable for routine determination of uromucoid in urine in described. The method is based on denaturation of uromucoid by sodium dodecyl sulphate at 37 degrees C before application on the immunoplates. A diffusion period of 30 h at 37 degrees C was chosen. The determination is uninfluenced by variations of urinary electrolytes, freezing and thawing, and storage at 4 degrees C for 3 weeks or at -20 degrees C for several months. The method can also be applied to lyophilized urines.
作为现有耗时技术的替代方法，本文描述了一种适用于常规测定尿中尿黏蛋白的径向免疫扩散法。该方法基于在将尿黏蛋白应用于免疫板之前，通过十二烷基硫酸钠在37℃使其变性。选择在37℃下扩散30小时。该测定不受尿电解质变化、冻融以及在4℃储存3周或在-20℃储存数月的影响。该方法也可应用于冻干尿液。
