Scheinberg M A, Cathcart E S
Immunology. 1974 Nov;27(5):953-63.
The functional capacity of lymphoid cells has been determined in amyloid-susceptible CBA/J and amyloid-resistant A/J mice. Following multiple casein injections mitogen responses to phytohaemagglutinin (PHA), concanavalin A (Con-A), pokeweed mitogen (PKW) and lipopolysaccharide (LPS) were significantly depressed in spleen cell suspensions of the A/J strain. Mitogen responses to PHA, Con-A and PKW were also reduced in casein-treated CBA/J animals, but improved during the active phase of amyloid deposition. The response to LPS was not affected in pre-amyloid and amyloid CBA/J animals. Spleen cells from caseintreated CBA/J mice responded normally to allogeneic cells from BALB/c and A/J mice; on the other hand graft-versus-host reactions in BALB/c mice receiving spleen cells from pre-amyloid and amyloid CBA/J mice were markedly impaired. The marked depression of certain T-cell populations, plus the maintenance of normal B-cell function in the CBA/J mice, suggest that disturbances of immunoregulatory mechanisms may be a critical step in the pathogenesis of experimental amyloid disease.
在易患淀粉样变性的CBA/J小鼠和抗淀粉样变性的A/J小鼠中测定了淋巴细胞的功能能力。多次注射酪蛋白后，A/J品系脾细胞悬液中对植物血凝素（PHA）、刀豆球蛋白A（Con-A）、商陆有丝分裂原（PKW）和脂多糖（LPS）的有丝分裂原反应显著降低。酪蛋白处理的CBA/J动物对PHA、Con-A和PKW的有丝分裂原反应也降低，但在淀粉样沉积的活跃期有所改善。在淀粉样变性前期和淀粉样变性的CBA/J动物中，对LPS的反应未受影响。酪蛋白处理的CBA/J小鼠的脾细胞对来自BALB/c和A/J小鼠的同种异体细胞反应正常；另一方面，接受来自淀粉样变性前期和淀粉样变性的CBA/J小鼠脾细胞的BALB/c小鼠的移植物抗宿主反应明显受损。CBA/J小鼠中某些T细胞群体的明显减少，加上B细胞功能的正常维持，表明免疫调节机制的紊乱可能是实验性淀粉样疾病发病机制中的关键步骤。
