Whitehead A S, Gonnerman W, Steel D M, Zahedi K
Division of Immunology, Children's Hospital, Boston, MA 02115.
Clin Exp Immunol. 1991 Apr;84(1):153-6.
An experimental model to assess the utility of variants of the serum amyloid P component (SAP) gene in predicting resistance to amyloidosis resulting from chronic inflammation was developed. F2 mice were generated from amyloid resistant (A/J) and amyloid susceptible (C57BL/6J) progenitor strains. Mice were injected daily with azocasein for 30 days, a treatment protocol determined to produce little amyloid deposition in the A/J progenitor strain and substantial amyloid deposition in the C57BL/6J progenitor strain. In a blind study the F2 spleens were subjectively scored for amyloid deposition and DNA was isolated from F2 livers and subjected to Southern blot analysis to determine the inheritance of progenitor strain specific SAP restriction fragment length polymorphisms (RFLPs). No correlation between relative amyloid resistance and SAP genotype was observed, indicating that SAP RFLPs have no value in predicting predisposition to amyloid disease in the mouse model studied.
建立了一个实验模型，以评估血清淀粉样蛋白P成分（SAP）基因变体在预测慢性炎症所致淀粉样变性抗性方面的效用。F2代小鼠由抗淀粉样蛋白（A/J）和易患淀粉样蛋白（C57BL/6J）的亲本品系培育而来。小鼠连续30天每天注射偶氮酪蛋白，该治疗方案确定为在A/J亲本品系中产生极少的淀粉样蛋白沉积，而在C57BL/6J亲本品系中产生大量的淀粉样蛋白沉积。在一项盲法研究中，对F2代小鼠的脾脏进行淀粉样蛋白沉积主观评分，并从F2代小鼠肝脏中分离DNA，进行Southern印迹分析，以确定亲本品系特异性SAP限制性片段长度多态性（RFLP）的遗传情况。未观察到相对淀粉样蛋白抗性与SAP基因型之间存在相关性，这表明在研究的小鼠模型中，SAP RFLP在预测淀粉样疾病易感性方面没有价值。
