一种用于展示凝胶沉淀人抗免疫球蛋白抗体的简单方法。
A simple method for demonstrating gel-precipitating human anti-immunoglobulin antibodies.
作者信息
Schalén C, Christensen P
出版信息
Acta Pathol Microbiol Scand C. 1977 Dec;85C(6):480-2. doi: 10.1111/j.1699-0463.1977.tb03672.x.
Abstract
The use of aggregated human IgG for the detection of anti-immunoglobulin antibodies by double immunodiffusion in gel is described. Of rheumatoid arthritis sera, 54 of 56 sera having a Waaler-Rose test titre of greater than or equal to 1:16 contained antibodies to aggregated IgG, in contrast to only 2 of 52 sera from blood donors. Furthermore, after 2-mercapto-ethanol treatment anti-IgG was still demonstrated in 5 of 16 sera.
摘要
本文描述了使用聚合人IgG通过凝胶双向免疫扩散法检测抗免疫球蛋白抗体。在类风湿性关节炎血清中，56份Waaler-Rose试验效价大于或等于1:16的血清中有54份含有抗聚合IgG抗体，相比之下，52份献血者血清中只有2份含有该抗体。此外，在2-巯基乙醇处理后，16份血清中有5份仍能检测到抗IgG。
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