通过吸光度变化研究孕酮诱导的大鼠肾溶酶体裂解
Progesterone-induced lysis of rat kidney lysosomes as studied by changes in light-absorbance.
作者信息
Badenoch-Jones P, Baum H
出版信息
Biochem J. 1974 Jul;142(1):1-6. doi: 10.1042/bj1420001.
Abstract
- A rat kidney lysosomal fraction was prepared by the method of Maunsbach (1966) and characterized by its content of representative marker enzymes for lysosomes, mitochondria, peroxisomes and endoplasmic reticulum. 2. It was shown that both pH-dependent and progesterone-induced lysis lead to a decrease in the E(520) of suspensions of this preparation. This decrease parallels quantitatively and temporally the release of soluble acid phosphatase. 3. It is suggested that E(520) measurements are a valid method for the continuous measurement of changes in lysosomal integrity. 4. As an example, results are included which demonstrate the ability of Zn(2+) to stabilize lysosomes against spontaneous and progesterone-induced lysis.
摘要
- 采用莫恩斯巴赫（1966年）的方法制备大鼠肾脏溶酶体组分，并通过其溶酶体、线粒体、过氧化物酶体和内质网代表性标志酶的含量进行表征。2. 结果表明，pH依赖性和孕酮诱导的裂解均导致该制剂悬浮液的E(520)降低。这种降低在数量和时间上与可溶性酸性磷酸酶的释放平行。3. 有人提出，E(520)测量是连续测量溶酶体完整性变化的有效方法。4. 作为一个例子，给出了结果，这些结果证明了Zn(2+)稳定溶酶体以抵抗自发和孕酮诱导裂解的能力。
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