内膜隔室的扩张及其与线粒体体积和离子转运的关系。
Expansion of the inner membrane compartment and its relation to mitochondrial volume and ion transport.
作者信息
Packer L, Wrigglesworth J M, Fortes P A, Pressman B C
出版信息
J Cell Biol. 1968 Nov;39(2):382-91. doi: 10.1083/jcb.39.2.382.
Abstract
Glutaraldehyde has been used to fix mitochondria undergoing rapid volume changes associated with energized ion transport under oscillatory state conditions and valinomycin-induced potassium uptake. Fixation was found to prevent structural changes which normally occur during ion accumulation or loss. By correlating packed volume measurements with electron microscopy, it is shown that changes in volume associated with ion movements reflect changes in the inner membrane compartment and that this compartment can be related to the sucrose inaccessible space. The method can therefore be used to accurately determine volume changes that arise from ion translocation.
摘要
戊二醛已被用于固定在振荡状态条件下以及缬氨霉素诱导的钾摄取过程中，与活跃的离子转运相关的、正在经历快速体积变化的线粒体。研究发现，固定作用可防止在离子积累或流失过程中正常发生的结构变化。通过将堆积体积测量结果与电子显微镜观察结果相关联，结果表明，与离子移动相关的体积变化反映了内膜隔室的变化，并且该隔室可能与蔗糖不可及空间有关。因此，该方法可用于准确测定由离子转运引起的体积变化。
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2
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3
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