在卵液中检测到的病毒复制刺激因子的生物学特性。

Biological characteristics of a stimulatory factor for viral replication detected in egg fluids.

作者信息

Eylan E, Gazit A

出版信息

Arch Virol. 1978;56(1-2):47-59. doi: 10.1007/BF01317282.

Abstract

Yolk sac and allantoic fluids and albumen from uninfected chicken eggs contain a low-molecular weight factor which, after 90 minutes of contact with cell cultures, significantly enhances viral replication. Of several viruses tested, Semliki Forest virus exhibited the highest (10(5)-fold) responsiveness to this enhancing factor. Maximal enhancement was obtained with cells subjected to low multiplicities of infection. The stimulation of viral replication was linear with the age of the cells in culture. The enhancing factor did not operate through an anti-interferon mechanism. However, it exerted a boosting effect on the low-grade cellular metabolism of ageing cell monolayers. Some of the physico-chemical features of the enhancing factor were determined and conjectures concerning its chemical makeup are discussed.

摘要

未感染的鸡蛋中的卵黄囊、尿囊液和蛋白含有一种低分子量因子，该因子与细胞培养物接触90分钟后，能显著增强病毒复制。在几种测试病毒中，Semliki森林病毒对这种增强因子的反应性最高（10^5倍）。用低感染复数的细胞可获得最大增强效果。病毒复制的刺激与培养细胞的年龄呈线性关系。增强因子不是通过抗干扰素机制起作用的。然而，它对衰老细胞单层的低水平细胞代谢有促进作用。确定了增强因子的一些物理化学特性，并讨论了关于其化学组成的推测。