Wallach D P
Prostaglandins. 1978 Apr;15(4):671-84. doi: 10.1016/0090-6980(78)90064-3.
Acetone-pentane powders of microsomal rich acetone precipitated fractions, have been prepared from hog aortas, ram seminal vesicles, and bovine corpora lutea. These preparations are all active in converting C14 labelled PGH2 to prostacyclin. The reaction was followed by quantitation of the spontaneous hydrolytic product, 6-keto PGF1alpha. The heat stability, pH optima, reactions with inhibitors, and other properties of these types are discussed. The comparative behavior of the respective enzyme preparations shows that while qualitatively they behave in a similar manner, quantitatively, there are significant differences between them, particularly with respect to heat treatment, and response to inhibitors.
已从猪主动脉、公羊精囊和牛黄体中制备了富含微粒体的丙酮沉淀级分的丙酮 - 戊烷粉末。这些制剂在将C14标记的PGH2转化为前列环素方面均具有活性。通过对自发水解产物6 - 酮 - PGF1α进行定量来跟踪反应。讨论了这些类型的热稳定性、最适pH值、与抑制剂的反应以及其他特性。各酶制剂的比较行为表明，虽然它们在定性上表现相似，但在定量上存在显著差异，特别是在热处理和对抑制剂的反应方面。
