趋化性研究。II. 正常血清对多种因子诱导趋化性的意义。
Studies on chemotaxis. II. The significance of normal sera for chemotaxis induced by various agents.
作者信息
Keller H U, Sorkin E
出版信息
Immunology. 1965 Nov;9(5):441-7.
Fresh normal sera of rabbits, guinea-pigs, pigs, calves, sheep and men when incubated with complexes of human serum albumin and rabbit antibody induce chemotaxis of rabbit granulocytes . No chemotaxis was observed when such sera were heated prior to the addition of antigen—antibody complexes. Incubation of fresh normal rabbit serum with heat-aggregated γ-globulin, PPD, glycogen, endotoxin or heat-killed results in formation of chemotactic factor(s). No chemotactic activity is found if these agents are incubated in inactivated normal rabbit serum or in serum-free medium. In contrast, the chemotactic activity of Witte's peptone does not depend on the presence of serum.
将人血清白蛋白与兔抗体的复合物与人、兔、豚鼠、猪、小牛及绵羊的新鲜正常血清一起温育时，可诱导兔粒细胞的趋化性。当在添加抗原-抗体复合物之前将此类血清加热时，则未观察到趋化性。将新鲜正常兔血清与热聚集的γ-球蛋白、结核菌素纯蛋白衍生物、糖原、内毒素或热杀死的细菌一起温育，可导致趋化因子的形成。如果将这些制剂在灭活的正常兔血清或无血清培养基中温育，则未发现趋化活性。相比之下，维特蛋白胨的趋化活性并不依赖于血清的存在。
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