关于分离的大鼠肝脏线粒体从铁蛋白中动员铁的研究。
Studies on the mobilization of iron from ferritin by isolated rat liver mitochondria.
作者信息
Ulvik R, Romslo I
出版信息
Biochim Biophys Acta. 1979 Dec 3;588(2):256-71. doi: 10.1016/0304-4165(79)90209-5.
Rat liver mitochondria and rat liver mitoplasts mobilize iron from ferritin by a mechanism which depends on a respiratory substrate (preferentially succinate), a small molecular weight electron mediator (FMN, phenazine methosulphate or methylene blue) and (near) anaerobic conditions. The release process under optimized conditions (approx. 50 mumol/1 FMN, 1 mmol/l succinate, 0.35 mmol/1 Fe(III) (as ferritin iron), 37 degrees C and pH 7.40) amounts to 0.9--1.2 nmol iron/mg protein per min. The results suggest that ferritin might function as an intermediate in the cytosolic transport of iron to the mitochondria.
大鼠肝脏线粒体和大鼠肝脏线粒体膜通过一种依赖于呼吸底物（优先为琥珀酸）、小分子电子介质（黄素单核苷酸、硫酸吩嗪甲酯或亚甲蓝）以及（接近）厌氧条件的机制从铁蛋白中动员铁。在优化条件下（约50 μmol/L黄素单核苷酸、1 mmol/L琥珀酸、0.35 mmol/L Fe(III)（以铁蛋白铁形式存在）、37℃和pH 7.40），释放过程达到每分钟0.9 - 1.2 nmol铁/毫克蛋白质。结果表明，铁蛋白可能作为铁从细胞质转运至线粒体过程中的一种中间体发挥作用。
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