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小鼠和兔珠蛋白及免疫球蛋白基因序列的电子显微镜分析。

Electron microscope analysis of mouse and rabbit globin and immunoglobulin gene sequences.

作者信息

Rochaix J D, Rougeon F, Mach B

出版信息

Gene. 1978 Feb;3(1):9-16. doi: 10.1016/0378-1119(78)90003-3.

Abstract

Mouse and rabbit globin and immunoglobulin gene sequences, which had been synthesized in vitro from eukaryotic mRNAs and inserted into plasmids, have been examined in the electron microscope. The size of the inserted beta rabbit and alpha and beta mouse globin DNA sequences has been estimated as 620 base pairs while the size of the inserted alpha rabbit globin DNA sequences was found to be about 490 base pairs. Heteroduplex analysis has revealed no structural abnormalities at the insertion sites of the chimeric plasmids except in the case of a plasmid containing an immunoglobulin light chain gene sequence of about 830 bases, in which 3 kb deletion adjacent to the insertion site was observed.

摘要

已对从小鼠和兔的真核生物mRNA体外合成并插入质粒的珠蛋白和免疫球蛋白基因序列进行了电子显微镜检查。已估计插入的兔β珠蛋白以及小鼠α和β珠蛋白DNA序列的大小为620个碱基对,而插入的兔α珠蛋白DNA序列的大小约为490个碱基对。异源双链分析表明,嵌合质粒的插入位点未发现结构异常,但含有约830个碱基的免疫球蛋白轻链基因序列的质粒除外,在该质粒中观察到插入位点附近有3 kb的缺失。

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