Stead A, Main J S, Ward M E, Watt P J
J Gen Microbiol. 1975 May;88(1):123-31. doi: 10.1099/00221287-88-1-123.
Lipopolysaccharides, extracted by phenol-water from five strains fo Neisseria gonorrhoeae, were purified by treatment with ribonuclease followed by multiple washes. These preparations were fatal to mice when administered in submicrogram amounts with actinomycin D, the LD50 values varying from 4 to 16 mug/kg. Analyses showed that all preparations contained glucose, galactose, glucosamine, heptose, 2-keto-3-deoxyoctonic acid and phosphate. All the lipopolysaccharides contained the same fatty acids, namely beta-OH-10:0, beta-OH-12:0, beta-OH-14:0, 12:0, 14:0,16:0, 16:1, 18:0 and 18:1. We were unable to detect significant differences between the lipopolysaccharides of virulent and avirulent gonococci or between penicillin-sensitive and resistant strains. Gonococcal lipopolysaccharides appeared to lack O-antigen side chains.
用酚水从五株淋病奈瑟菌中提取脂多糖，先用核糖核酸酶处理，然后多次洗涤进行纯化。当与放线菌素D一起以亚微克量给药时，这些制剂对小鼠是致命的，半数致死量（LD50）值在4至16微克/千克之间。分析表明，所有制剂都含有葡萄糖、半乳糖、葡糖胺、庚糖、2-酮-3-脱氧辛酸和磷酸盐。所有脂多糖都含有相同的脂肪酸，即β-羟基-10:0、β-羟基-12:0、β-羟基-14:0、12:0、14:0、16:0、16:1、18:0和18:1。我们未能检测到有毒和无毒淋球菌的脂多糖之间或青霉素敏感和耐药菌株之间的显著差异。淋球菌脂多糖似乎缺乏O抗原侧链。
