Alishiri Romina, Zeinali Reza, Pourtaghi Kimia, Heshmatipour Zoheir, Rahimi Azam, Heidari-Keshel Saeed, Zaeifi Davood, Sefat Farshid, Biazar Esmaeil
Department of Biomedical Engineering, To.C., Islamic Azad University, Tonekabon, Iran.
Group of Molecular and Industrial Biotechnology, Department of Chemical Engineering, Universität Politècnica de Catalunya, Terrassa, Spain.
ChemistryOpen. 2026 Feb;15(2):e202500539. doi: 10.1002/open.202500539.
Hydrogel adhesives have attracted significant attention for diverse biomedical applications, including tissue engineering, wound dressings, crack propagation prevention, and hemorrhage control. In this study, a tissue adhesive composed of polyethylene glycol, tannic acid, and gelatin, with different concentrations of silk fibroin (SF), was synthesized and systematically evaluated using fourier transform infrared, scanning electron microscopy (SEM), adhesion strength tests, swelling and degradation analysis, cytotoxicity and proliferation studies and antibacterial assays. Structural analysis confirmed the presence of strong hydrogen bonds among the components, while SEM imaging revealed a porous morphology for all samples. Mechanical testing demonstrated that the incorporation of SF additive significantly influenced the adhesion strength of the hydrogels. Furthermore, an increase in SF content led to a significant reduction in swelling capacity and degradation rate. The adhesive samples exhibited excellent biocompatibility, and antibacterial assays indicated that the SF additive maintained antibacterial efficacy comparable to the samples without the additive. These findings highlight the synthesized tissue adhesive as a promising candidate with favorable mechanical and biological properties for potential clinical applications in tissue engineering and wound healing.
水凝胶粘合剂因其在多种生物医学应用中的潜力而备受关注，这些应用包括组织工程、伤口敷料、防止裂纹扩展和控制出血。在本研究中，合成了一种由聚乙二醇、单宁酸和明胶组成，并含有不同浓度丝素蛋白（SF）的组织粘合剂，并通过傅里叶变换红外光谱、扫描电子显微镜（SEM）、粘附强度测试、溶胀和降解分析、细胞毒性和增殖研究以及抗菌试验对其进行了系统评估。结构分析证实了各组分之间存在强氢键，而SEM成像显示所有样品均具有多孔形态。力学测试表明，SF添加剂的加入显著影响了水凝胶的粘附强度。此外，SF含量的增加导致溶胀能力和降解速率显著降低。粘合剂样品表现出优异的生物相容性，抗菌试验表明，SF添加剂保持了与不含添加剂的样品相当的抗菌效果。这些发现突出了合成的组织粘合剂作为一种有前途的候选材料，具有良好的机械和生物学性能，并有望在组织工程和伤口愈合中得到临床应用。
