Röder A, Pünter J
Zentralbl Bakteriol Orig A. 1977 Dec;239(4):459-64.
The influence of NPV on mammals was tested in animals and tissue cultures. Toxicity experiments were performed in rats: per os application (3 X 10(9) Virusinclusionbodies per kg body weight) and intravenous (5 X 10(8) Virusinclusionbodies per kg body weight). Skin irrition was performed in guinea pigs. In each tested animal no virus induced changes could be shown. Tissue culture studies were done in tissues of human (Hela Ohio), monkey (Vero) and rats (REC). The infectious unit was Ac NPV derived from infected, virus-producing insect tissue cultures. Three blind passages were done in each examined cell type and no signs of viral replication could be shown. An additional infectivity assay of cellfree extracts in insect cell cultures as indicator system remained negative.
在动物和组织培养中测试了NPV对哺乳动物的影响。在大鼠身上进行了毒性实验：经口给药（每千克体重3×10⁹个病毒包涵体）和静脉注射（每千克体重5×10⁸个病毒包涵体）。在豚鼠身上进行了皮肤刺激实验。在每只受试动物中均未发现病毒诱导的变化。在人（Hela Ohio）、猴（Vero）和大鼠（REC）的组织中进行了组织培养研究。感染单位是来自感染的、产生病毒的昆虫组织培养物的Ac NPV。在每种检测的细胞类型中进行了三次盲传，未发现病毒复制的迹象。以昆虫细胞培养物作为指示系统对无细胞提取物进行的额外感染性测定结果仍为阴性。
