Henneberg G, Heller S, Jordanski H
Zentralbl Bakteriol A. 1980 Mar;246(2):167-83.
Infrared radiation and 1-2 micron microwaves influenced the infectivity of Newcastle Disease Virus (NDV) upon chick embryo tracheal tissue in such a way that the expected destruction of ciliated epithelium turned out to be incomplete or did not take place at all, respectively. Due to the fact that direct damaging of viruses following such radiation was not shown and that it was our intention to arrange the experimental set-up as if natural rhinolaryngeal conditions had induced the infection of the mucous membrane of the respiratory tract a study was performed in order to establish the radiation influence on mediator substances involved in the virus infection of mucous membranes. The mucopolysaccharides, chondroitine sulphate and hyaluronic acid, as well as mucin served as models; in addition protamine sulphate was used for reasons of comparison. The influence of the above substances upon NDV-infection in organ cultures and the effect of electromagnetic waves upon such influence were studied. By choosing a virus concentration of 10(-8)/ml on chick embryo tracheal tissue it was established that the application of infrared radiation (Osram Siccatherm Infrared Radiator,- 1-2 micron) and cm-waves (Klystron Raytheon Comp. Mass. USA, - 1.35 cm) for a length of 10 min. inhibited Newcastle Disease Virus (NDV) infectivity. The suspension fluid was treated with infrared and the tissue with cm-waves. Previous experiments revealed that direct radiation influence upon viruses cannot be taken for granted which is why the agents, chondroitine sulphate (polyanion) 5-10 microgram/ml, and hyaluronic acid (polyanion) 10-50 microgram/ml, were used in order to study such influence upon NDV-infected tracheal mucous membrane. In addition, protamine sulphate (polycation), 5-10 microgram/ml, and mucin were used. All the above mentioned substances influenced viral infectivity in organ cultures-expressed in terms of quotients: quotient 1 means no influence on the motility of the ciliated mucous membrane cells and quotient 0.01 means the complete destruction of the cells. Chondroitine sulphate inhibited strong but promoted weak virus infectivity, hyaluronic acid inhibited strong virus infectivity, protamine sulphate inhibited strong but promoted weak virus infectivity and mucin promoted weak virus infectivity in accordance with the used virus concentrations (Table 2). Under the influence of infrared radiation the mediator substances exercised a different influence upon viral infectivity: 25 times out of 32 experiments chondroitine sulphate was inhibited and hyaluronic acid increased in 8 out of 12 experiments (Table 2). The influence of protamine sulphate on viral infectivity was found to be increased 39 times and inhibited 14 times out of 54 experiments, mucin was inhibited 6 times. Furthermore, it is to be added that additional radiation with cm-waves increased the influence of infrared radiation (Table 1)...
红外线辐射和1 - 2微米的微波以这样的方式影响新城疫病毒（NDV）对鸡胚气管组织的感染性，即预期的纤毛上皮破坏分别变得不完全或根本没有发生。由于未显示出此类辐射后病毒受到直接损伤，且我们的意图是设置实验装置，使其仿佛是自然鼻喉状况引发了呼吸道黏膜感染，因此进行了一项研究，以确定辐射对参与黏膜病毒感染的介质物质的影响。黏多糖、硫酸软骨素和透明质酸以及黏蛋白被用作模型；此外，出于比较目的使用了硫酸鱼精蛋白。研究了上述物质对器官培养中NDV感染的影响以及电磁波对此类影响的作用。通过在鸡胚气管组织上选择10^(-8)/ml的病毒浓度，确定施加10分钟的红外线辐射（欧司朗Siccatherm红外线辐射器，- 1 - 2微米）和厘米波（美国马萨诸塞州雷神公司的速调管，- 1.35厘米）会抑制新城疫病毒（NDV）的感染性。悬浮液用红外线处理，组织用厘米波处理。先前的实验表明，不能想当然地认为辐射对病毒有直接影响，这就是为什么使用硫酸软骨素（聚阴离子）5 - 10微克/毫升和透明质酸（聚阴离子）10 - 50微克/毫升来研究它们对NDV感染的气管黏膜的影响。此外，还使用了硫酸鱼精蛋白（聚阳离子）5 - 10微克/毫升和黏蛋白。所有上述物质都影响器官培养中的病毒感染性，用商数表示：商数1表示对纤毛黏膜细胞的运动性无影响，商数0.01表示细胞完全破坏。硫酸软骨素抑制强病毒感染性但促进弱病毒感染性，透明质酸抑制强病毒感染性，硫酸鱼精蛋白抑制强病毒感染性但促进弱病毒感染性，黏蛋白根据所用病毒浓度促进弱病毒感染性（表2）。在红外线辐射的影响下，介质物质对病毒感染性产生了不同的影响：在32次实验中有25次硫酸软骨素受到抑制，在12次实验中有8次透明质酸增加（表2）。在54次实验中，发现硫酸鱼精蛋白对病毒感染性的影响增加了39次，受到抑制14次，黏蛋白受到抑制6次。此外，还应补充的是，额外的厘米波辐射增加了红外线辐射的影响（表1）……
