Mossel D A, van Ekeren A J, Eelderink I
Zentralbl Bakteriol Orig B. 1977 Dec;165(5-6):498-516.
A new method is described which can be used for the examination of piped drinking water. It is also suitable for monitoring water which was initially of potable quality, and is intended for reuse in the food industry. The method is based on CLARK's "P-A test" and, because this allows many bacterial types to be detected, i.e. Enterobacteriaceae, E. coli, P. aeruginosa, Aeromonadaceae and LANCEFIELD group D streptococci it is called differential hydrobacteriogramme. A preliminary resuscitation treatment to revive sublethally injured cells is essential in this procedure. In earlier work this was attained by adding an equal volume of double strength nutrient broth and later double strength MACCONKEY purple broth, making the method somewhat bulky. In the new procedure, after the resuscitation step, a concentrated bile salts/indicator solution is added, allowing subsequent selective enrichment of the taxa sought. Positive enrichment cultures are examined for these organisms by the procedures summarized in Fig. 1. The new method, when tested on approx. 150 artificially inoculated and 92 natural samples, showed the same productivity and selectivity as the one introduced earlier. The new method is recommended for routine monitoring purposes, because it is less bulky.
本文描述了一种可用于检测管道饮用水的新方法。它也适用于监测最初符合饮用水质量标准、打算在食品工业中再利用的水。该方法基于克拉克的“P-A试验”，由于它能检测多种细菌类型，即肠杆菌科、大肠杆菌、铜绿假单胞菌、气单胞菌科和D群链球菌，因此被称为鉴别性水细菌图谱。在此过程中，对亚致死损伤细胞进行初步复苏处理至关重要。在早期的工作中，这是通过加入等体积的双倍强度营养肉汤，后来又加入双倍强度麦康凯紫色肉汤来实现的，这使得该方法有些繁琐。在新方法中，复苏步骤后加入浓缩胆盐/指示剂溶液，以便随后对所需菌群进行选择性富集。通过图1总结的程序对阳性富集培养物进行这些微生物的检测。新方法在大约150个人工接种样本和92个自然样本上进行测试时，显示出与早期引入的方法相同的效率和选择性。由于新方法不那么繁琐，因此推荐用于常规监测。
