干酪乳杆菌血清叶酸测定的连续流动自动化
Continuous-flow automation of the Lactobacillus casei serum folate assay.
作者信息
Tennant G B
出版信息
J Clin Pathol. 1977 Dec;30(12):1168-74. doi: 10.1136/jcp.30.12.1168.
A method is described for the continuous-flow automation of the serum folate assay using Lactobacillus casei. The total incubation period is approximately four hours. The growth response of the organism to folate is estimated by measuring the rate of reduction of 2,3,5-triphenyl tetrazolium chloride (TTC). A simple continuous culture apparatus is used to grow the inoculum. Supplementation of the assay medium is necessary to obtain parallel results. A statistical assessment shows a favourable comparison with the whole-serum tube assay using a chloramphenicol resistant strain of L. casei. The method is less sensitive to inhibitory substances than the tube assay.
描述了一种使用干酪乳杆菌对血清叶酸测定进行连续流动自动化的方法。总孵育期约为四小时。通过测量氯化三苯基四氮唑（TTC）的还原速率来估计该生物体对叶酸的生长反应。使用简单的连续培养装置来培养接种物。为获得平行结果，测定培养基的补充是必要的。统计评估表明，与使用对氯霉素耐药的干酪乳杆菌菌株进行的全血清试管测定相比，该方法具有良好的比较效果。该方法比试管测定对抑制物质的敏感性更低。
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