Yoshida T, Mauger A, Weissbach H, Katz E
J Bacteriol. 1968 Mar;95(3):952-8. doi: 10.1128/jb.95.3.952-958.1968.
The inhibitory effect of methylprolines on actinomycin biosynthesis by Streptomyces antibioticus was studied; the order of effectiveness was 3- >4- >5-methyl-dl-proline. Cis-3-methyl-dl-proline was 14 times more active than the trans isomer. It was also found that 4- and, possibly, 5-methylproline were incorporated into the actinomycin molecule. When 4-methylproline was present, three new actinomycins, representing 50 to 60% of the antibiotic mixture, were synthesized. Growth of the organism may be stimulated at concentrations (0.1 to 1.0 mug per ml) of 3-methylproline that inhibit antibiotic formation, thus providing additional evidence for a different mechanism of actinomycin synthesis from that of protein synthesis. Azetidine-2-carboxylic acid, piperdine-2-carboxylic acid, and hydroxyproline (but not sarcosine) reversed the inhibition due to 3-methylproline.
研究了甲基脯氨酸对产抗生素链霉菌合成放线菌素的抑制作用；其有效性顺序为3->4->5-甲基-dl-脯氨酸。顺式-3-甲基-dl-脯氨酸的活性比反式异构体高14倍。还发现4-甲基脯氨酸以及可能的5-甲基脯氨酸被掺入放线菌素分子中。当存在4-甲基脯氨酸时，合成了三种新的放线菌素，占抗生素混合物的50%至60%。在抑制抗生素形成的3-甲基脯氨酸浓度（每毫升0.1至1.0微克）下，生物体的生长可能会受到刺激，从而为放线菌素合成与蛋白质合成机制不同提供了额外证据。氮杂环丁烷-2-羧酸、哌啶-2-羧酸和羟脯氨酸（但不包括肌氨酸）可逆转3-甲基脯氨酸引起的抑制作用。
