Golub E E, Ward M A, Nishimura J S
J Bacteriol. 1969 Nov;100(2):977-84. doi: 10.1128/jb.100.2.977-984.1969.
Actinomycin synthesis by washed mycelia of Streptomyces antibioticus has been conducted in the presence of 3-hydroxy-4-methylanthranilate-(carboxyl-(14)C). Incorporation of this compound into actinomycins has been observed, which constitutes further evidence that 3-hydroxy-4-methylanthranilate is an intermediate in actinomycin biosynthesis. The position of the incorporated label has been determined to be within the actinomycin chromophore, and the label appears to be equally distributed between both halves of the chromophore. Incidental to these findings was the observation that the (14)C-labeled actinomycins were subject to rapid reabsorption by the organism with actinomycin V taken up preferentially to actinomycin IV.
在3-羟基-4-甲基邻氨基苯甲酸-（羧基-¹⁴C）存在的情况下，对产抗生素链霉菌的洗涤菌丝体进行了放线菌素合成实验。已观察到该化合物掺入放线菌素中，这进一步证明3-羟基-4-甲基邻氨基苯甲酸是放线菌素生物合成的中间体。已确定掺入标记的位置在放线菌素发色团内，并且标记似乎在发色团的两半之间均匀分布。这些发现附带的一个观察结果是，¹⁴C标记的放线菌素会被该生物体快速重新吸收，其中放线菌素V比放线菌素IV更优先被吸收。
