Popenko V I, Vengerov Y Y
Mol Biol Rep. 1978 Feb 28;4(1):45-50. doi: 10.1007/BF00775180.
Chromatin from calf thymus isolated under hypotonic conditions in the presence of various agents was investigated by methods of electron microscopy prior to and after EDTA treatment. It is shown that the presence of chelating agents and, especially, the application of considerable mechanical forces in the course of isolation may cause damage to the nucleosomal structure of the chromatin. Moreover, sufficiently great mechanical forces are liable to destroy the structure of the chromatin nucleosomal fibres even when they are packed in structures of a higher order of organization of the chromatin.
在低渗条件下，于各种试剂存在的情况下分离得到的小牛胸腺染色质，在进行EDTA处理前后，通过电子显微镜方法进行了研究。结果表明，螯合剂的存在，尤其是在分离过程中施加相当大的机械力，可能会对染色质的核小体结构造成损伤。此外，即使染色质核小体纤维堆积在染色质更高层次的组织结构中，足够大的机械力也容易破坏其结构。
