Whitaker J N, Starr J L
J Clin Invest. 1968 Jul;47(7):1496-510. doi: 10.1172/JCI105843.
Blood samples containing antibodies to DNA were obtained from patients with systemic lupus erythematosus (SLE) and rabbits immunized with denatured DNA complexed to methylated bovine serum albumin. The immunoglobulin fractions from these sources did not decrease the over-all template activity of singlestranded DNA with DNA polymerase or DNA-dependent RNA polymerase. In competition studies, both DNA polymerase and DNA-dependent RNA polymerase inhibited the binding of DNA antibodies to single-stranded DNA, as evidenced by inhibition of micro-complement fixation. These findings suggest that antibodies to DNA fail to decrease denatured DNA template activity because the enzymes which use a single-stranded DNA template can displace or block the antibodies from the denatured DNA as a result of greater binding affinity to the denatured DNA. The anti-DNA antibodies associated with SLE, therefore, may not be involved in the pathogenesis of the intracellular abnormalities associated with the disease.
从系统性红斑狼疮（SLE）患者以及用与甲基化牛血清白蛋白复合的变性DNA免疫的兔子身上获取了含有抗DNA抗体的血样。这些来源的免疫球蛋白组分不会降低单链DNA在DNA聚合酶或依赖DNA的RNA聚合酶作用下的总体模板活性。在竞争研究中，DNA聚合酶和依赖DNA的RNA聚合酶均抑制了DNA抗体与单链DNA的结合，微量补体结合抑制实验证明了这一点。这些发现表明，抗DNA抗体未能降低变性DNA模板活性，是因为使用单链DNA模板的酶由于对变性DNA具有更高的结合亲和力，能够将抗体从变性DNA上置换或阻断。因此，与SLE相关的抗DNA抗体可能不参与该疾病相关的细胞内异常的发病机制。
